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Reaction mixtures were incubated for 2 h at 37 C, then terminated by the addition of DTT-containing SDS sample buffer, followed by immunoblot analysis

remain upright for 2-3 hours post-meal Identify and avoid personal trigger foods that worsen symptoms Medication timing and administration: Administer Zepbound at a consistent time weekly, preferably when you can rest if needed Some patients find evening administration allows them to "sleep through" peak side effects Ensure proper injection technique and site rotation to optimize absorption Pharmacological interventions: Consult your healthcare provider before adding any medications for nausea management

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Rotate by day, evening or treatment session